ART: A machine learning Automated Recommendation Tool for synthetic biology

Synthetic biology allows us to bioengineer cells to synthesize novel valuable molecules such as renewable biofuels or anticancer drugs. However, traditional synthetic biology approaches involve ad-hoc non systematic engineering practices, which lead to long development times. Here, we present the Automated Recommendation Tool ( ART), a tool that leverages machine learning and probabilistic modeling techniques to guide synthetic biology in a systematic fashion, without the need for a full mechanistic understanding of the biological system. Using sampling-based optimization, ART provides a set of recommended strains to be built in the next engineering cycle, alongside probabilistic predictions of their production levels. We demonstrate the capabilities of ART on simulated and real data sets and discuss possible difficulties in achieving satisfactory predictive power. 2 Introduction Metabolic engineering 1 enables us to bioengineer cells to synthesize novel valuable molecules such as renewable biofuels 2,3 or anticancer drugs.