Towards Automated Design of Riboswitches

Using computational (Vorobyeva et al., 2018) for a single target, resulting methods to reduce the number of candidates in expensive, time-consuming, and inefficient screens. It for the screen could drastically decrease is common knowledge that the length, the secondary structures, these costs. However, existing computational approaches their diversity, and the nucleotide composition of the do not fully satisfy all requirements for sequences in the initial library are crucial for a successful the design of such initial screening libraries. In SELEX (Vorobyeva et al., 2018; Kohlberger & Gadermaier, this work, we present a new method, libLEARNA, 2022). However, random libraries of fixed-length sequences capable of providing RNA focus libraries of diverse are still most widely used. Focused design can help to decrease variable-length qualified candidates. Our the size of the initial library and therefore drastically novel structure-based design approach considers reduce the costs of these SELEX pipelines.