Using 3D reconstruction from image motion to predict total leaf area in dwarf tomato plants

Accurate estimation of total leaf area (TLA) is essential for assessing plant growth, photosynthetic activity, and transpiration but remains a challenge for bushy plants like dwarf tomatoes. Traditional destructive methods and imaging-based techniques often fall short due to labor intensity, plant damage, or the inability to capture complex canopies. This study evaluated a non-destructive method combining sequential 3D reconstructions from RGB images and machine learning to estimate TLA for three dwarf tomato cultivars-- Mohamed, Hahms Gelbe Topftomate, and Red Robin--grown under controlled greenhouse conditions. Two experiments, conducted in spring-summer and autumn-winter, included 73 plants, yielding 418 TLA measurements using an "onion" approach, where layers of leaves were sequentially removed and scanned. High-resolution videos were recorded from multiple angles for each plant, and 500 frames were extracted per plant for 3D reconstruction. Point clouds were created and processed, four reconstruction algorithms (Alpha Shape, Marching Cubes, Poisson's, and Ball Pivoting) were tested, and meshes were evaluated using seven regression models: Multivariable Linear Regression (MLR), Lasso Regression (Lasso), Ridge Regression (Ridge-Reg), Elastic Net Regression (ENR), Random Forest (RF), extreme gradient boosting (XGBoost), and Multilayer Perceptron (MLP). The Alpha Shape reconstruction (α = 3) combined with XGBoost yielded the best performance, achieving an R of 0.80 and MAE of 489 cm These findings demonstrate the robustness of our approach across variable environmental conditions and canopy structures. This scalable, automated TLA estimation method is particularly suited for urban farming and precision agriculture, offering practical implications for automated pruning, improved resource efficiency, and sustainable food production. Keywords: Total leaf area, dwarf tomato, point cloud, mesh reconstruction, machine learning, precision agriculture 1. Introduction Total leaf area (TLA) is a comprehensive metric describing the plant's growth and functioning. It is a primary metric that describes the plant's photosynthetic activity and transpiration capacity. Normalized by the plant's surface area, TLA may provide information on the canopy structure, which is crucial for understanding the plant's energy and resource efficiency. For example, reduced TLA is a sign of stress (Dong et al., 2019), while excessive biomass, indicated by a higher TLA, signifies lower water use efficiency (Glenn et al., 2006). Farmers often use pruning to reduce TLA in commercial crops to increase crop productivity (Budiarto et al., 2023). However, measuring and finding the optimum TLA of the crop are challenging tasks.