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Object tracking --following objects over time --is an essential image analysis technique used to quantify dynamic processes in biosciences. A new application called TrackMate v7 enables scientists to track objects in images easily. TrackMate is a free, open-source tool available as part of the Fiji image analysis platform. In life sciences, tracking is used, for instance, to follow the movement of molecules, subcellular organelles, bacteria, cells, and whole animals. However, due to the sheer diversity of images used in research, no single application can address every tracking challenge.

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