Automated Pollen Recognition in Optical and Holographic Microscopy Images

Abstract--This study explores the application of deep learning to improve and automate pollen grain detection and classification in both optical and holographic microscopy images, with a particular focus on veterinary cytology use cases. We used YOLOv8s for object detection and MobileNetV3L for the classification task, evaluating their performance across imaging modalities. The models achieved 91.3% mAP50 for detection and 97% overall accuracy for classification on optical images, whereas the initial performance on greyscale holographic images was substantially lower . We addressed the performance gap issue through dataset expansion using automated labeling and bounding box area enlargement. These techniques, applied to holographic images, improved detection performance from 2.49% to 13.3% mAP50 and classification performance from 42% to 54%. Our work demonstrates that, at least for image classification tasks, it is possible to pair deep learning techniques with cost-effective lensless digital holographic microscopy devices. I. INTRODUCTION Microscopy is an integral part of most veterinary medicine diagnostic procedures.