Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics

We thank Twist Bioscience for synthesizing and cloning ChR sequences, D. Wagenaar (California Institute of Technology) and the Caltech Neurotechnology Center for building the mouse treadmill, J. Brake (California Institute of Technology) for performing spectrometer measurements, J. Bedbrook for critical reading of the manuscript and the Gradinaru and Arnold laboratories for helpful discussions. This work was funded by the Institute for Collaborative Biotechnologies grant no. W911NF-09-0001 from the US Army Research Office (F.H.A) and the National Institutes of Health (NIH) (V.G.): NIH BRAIN grant no. RF1MH117069, NIH Director's Pioneer Award grant no. DP1NS111369, NIH Director's New Innovator Award grant no.