supp

Dataset documentation and intended uses: 1. Introduction The current methodologies for enzyme annotation primarily rely on established databases and classifications such as KEGG Orthology (KO), Enzyme Commission (EC) numbers, and Gene Ontology (GO) annotations, each with its specific focus and methodology. For instance, the EC system categorizes enzymes based on the chemical reactions they catalyze, providing a hierarchical numerical classification. KO links gene products to their functional orthologs across different species, whereas GO offers a broader ontology for describing the roles of genes and proteins in any organism. Despite their widespread use, these systems have notable limitations. The EC classification, while widely used, sometimes groups vastly different enzymes under the same category or subdivides similar ones excessively, based on the substrates they interact with--leading to ambiguities in enzyme function characterization.