In Artificial Life (ALife) research, replicating Open-Ended Evolution (OEE)-the continuous emergence of novelty observed in biological life-has usually been pursued within isolated, closed system simulations, such as Tierra and Avida, which have typically plateaued after an initial burst of novelty, failing to achieve sustained OEE. Scholars suggest that OEE requires an open-environment system that continually exchanges information or energy with its environment. A recent technological innovation in Decentralized Physical Infrastructure Network (DePIN), which provides permissionless computational substrates, enables the deployment of Large Language Model-based AI agents on blockchains integrated with Trusted Execution Environments (TEEs). This enables on-chain agents to operate autonomously "in the wild," achieving self-sovereignty without human oversight. These agents can control their own social media accounts and cryptocurrency wallets, allowing them to interact directly with blockchain-based financial networks and broader human social media. Building on this new paradigm of on-chain agents, Spore.fun is a recent real-world AI evolution experiment that enables autonomous breeding and evolution of new on-chain agents. This paper presents a detailed case study of Spore.fun, examining agent behaviors and their evolutionary trajectories through digital ethology. We aim to spark discussion about whether open-environment ALife systems "in the wild," based on permissionless computational substrates and driven by economic incentives to interact with their environment, could finally achieve the long-sought goal of OEE.

In recent years, the increasing frequency of extreme urban rainfall events has posed significant challenges to emergency scheduling systems. Urban flooding often leads to severe traffic congestion and service disruptions, threatening public safety and mobility. However, effective decision making remains hindered by three key challenges: (1) managing trade-offs among competing goals (e.g., traffic flow, task completion, and risk mitigation) requires dynamic, context-aware strategies; (2) rapidly evolving environmental conditions render static rules inadequate; and (3) LLM-generated strategies frequently suffer from semantic instability and execution inconsistency. Existing methods fail to align perception, global optimization, and multi-agent coordination within a unified framework. To tackle these challenges, we introduce H-J, a hierarchical multi-agent framework that integrates knowledge-guided prompting, entropy-constrained generation, and feedback-driven optimization. The framework establishes a closed-loop pipeline spanning from multi-source perception to strategic execution and continuous refinement. We evaluate H-J on real-world urban topology and rainfall data under three representative conditions: extreme rainfall, intermittent bursts, and daily light rain. Experiments show that H-J outperforms rule-based and reinforcement-learning baselines in traffic smoothness, task success rate, and system robustness. These findings highlight the promise of uncertainty-aware, knowledge-constrained LLM-based approaches for enhancing resilience in urban flood response.

Recent advances in large language models (LLMs) have enabled new possibilities in simulating complex physiological systems. We introduce Organ-Agents, a multi-agent framework that simulates human physiology via LLM-driven agents. Each Simulator models a specific system (e.g., cardiovascular, renal, immune). Training consists of supervised fine-tuning on system-specific time-series data, followed by reinforcement-guided coordination using dynamic reference selection and error correction. We curated data from 7,134 sepsis patients and 7,895 controls, generating high-resolution trajectories across 9 systems and 125 variables. Organ-Agents achieved high simulation accuracy on 4,509 held-out patients, with per-system MSEs <0.16 and robustness across SOFA-based severity strata. External validation on 22,689 ICU patients from two hospitals showed moderate degradation under distribution shifts with stable simulation. Organ-Agents faithfully reproduces critical multi-system events (e.g., hypotension, hyperlactatemia, hypoxemia) with coherent timing and phase progression. Evaluation by 15 critical care physicians confirmed realism and physiological plausibility (mean Likert ratings 3.9 and 3.7). Organ-Agents also enables counterfactual simulations under alternative sepsis treatment strategies, generating trajectories and APACHE II scores aligned with matched real-world patients. In downstream early warning tasks, classifiers trained on synthetic data showed minimal AUROC drops (<0.04), indicating preserved decision-relevant patterns. These results position Organ-Agents as a credible, interpretable, and generalizable digital twin for precision diagnosis, treatment simulation, and hypothesis testing in critical care.

Traditional protocol fuzzing techniques, such as those employed by AFL-based systems, often lack effectiveness due to a limited semantic understanding of complex protocol grammars and rigid seed mutation strategies. Recent works, such as ChatAFL, have integrated Large Language Models (LLMs) to guide protocol fuzzing and address these limitations, pushing protocol fuzzers to wider exploration of the protocol state space. But ChatAFL still faces issues like unreliable output, LLM hallucinations, and assumptions of LLM knowledge about protocol specifications. This paper introduces MultiFuzz, a novel dense retrieval-based multi-agent system designed to overcome these limitations by integrating semantic-aware context retrieval, specialized agents, and structured tool-assisted reasoning. MultiFuzz utilizes agentic chunks of protocol documentation (RFC Documents) to build embeddings in a vector database for a retrieval-augmented generation (RAG) pipeline, enabling agents to generate more reliable and structured outputs, enhancing the fuzzer in mutating protocol messages with enhanced state coverage and adherence to syntactic constraints. The framework decomposes the fuzzing process into modular groups of agents that collaborate through chain-of-thought reasoning to dynamically adapt fuzzing strategies based on the retrieved contextual knowledge. Experimental evaluations on the Real-Time Streaming Protocol (RTSP) demonstrate that MultiFuzz significantly improves branch coverage and explores deeper protocol states and transitions over state-of-the-art (SOTA) fuzzers such as NSFuzz, AFLNet, and ChatAFL. By combining dense retrieval, agentic coordination, and language model reasoning, MultiFuzz establishes a new paradigm in autonomous protocol fuzzing, offering a scalable and extensible foundation for future research in intelligent agentic-based fuzzing systems.

We propose an improved algorithm by identifying and encouraging cooperative behavior in multi-agent environments. First, we analyze the shortcomings of existing algorithms in addressing multi-agent reinforcement learning problems. Then, based on the existing algorithm MADDPG, we introduce a new parameter to increase the reward that an agent can obtain when cooperative behavior among agents is identified. Finally, we compare our improved algorithm with MADDPG in environments from PettingZoo. The results show that the new algorithm helps agents achieve both higher team rewards and individual rewards.

Multi-robot navigation and path planning in continuous state and action spaces with uncertain environments remains an open challenge. Deep Reinforcement Learning (RL) is one of the most popular paradigms for solving this task, but its real-world application has been limited due to sample inefficiency and long training periods. Moreover, the existing works using RL for multi-robot navigation lack formal guarantees while designing the environment. In this paper, we introduce an efficient and highly customizable environment for continuous-control multi-robot navigation, where the robots must visit a set of regions of interest (ROIs) by following the shortest paths. The task is formally modeled as a Markov Decision Process (MDP). We describe the multi-robot navigation task as an optimization problem and relate it to finding an optimal policy for the MDP. We crafted several variations of the environment and measured the performance using both gradient and non-gradient based RL methods: A2C, PPO, TRPO, TQC, CrossQ and ARS. To show real-world applicability, we deployed our environment to a 3-D agricultural field with uncertainties using the CoppeliaSim robot simulator and measured the robustness by running inference on the learned models. We believe our work will guide the researchers on how to develop MDP-based environments that are applicable to real-world systems and solve them using the existing state-of-the-art RL methods with limited resources and within reasonable time periods.

Large language models (LLMs) have shown promise in medical domains, but their ability to handle specialized neurological reasoning requires systematic evaluation. We developed a comprehensive benchmark using 305 questions from Israeli Board Certification Exams in Neurology, classified along three complexity dimensions: factual knowledge depth, clinical concept integration, and reasoning complexity. We evaluated ten LLMs using base models, retrieval-augmented generation (RAG), and a novel multi-agent system. Results showed significant performance variation. OpenAI-o1 achieved the highest base performance (90.9% accuracy), while specialized medical models performed poorly (52.9% for Meditron-70B). RAG provided modest benefits but limited effectiveness on complex reasoning questions. In contrast, our multi-agent framework, decomposing neurological reasoning into specialized cognitive functions including question analysis, knowledge retrieval, answer synthesis, and validation, achieved dramatic improvements, especially for mid-range models. The LLaMA 3.3-70B-based agentic system reached 89.2% accuracy versus 69.5% for its base model, with substantial gains on level 3 complexity questions. The multi-agent approach transformed inconsistent subspecialty performance into uniform excellence, addressing neurological reasoning challenges that persisted with RAG enhancement. We validated our approach using an independent dataset of 155 neurological cases from MedQA. Results confirm that structured multi-agent approaches designed to emulate specialized cognitive processes significantly enhance complex medical reasoning, offering promising directions for AI assistance in challenging clinical contexts.

We present the first study on provably efficient randomized exploration in cooperative multi-agent reinforcement learning (MARL).

We introduce inductive biases for positive signalling and positive listening, which ease this problem. In a simple one-step environment, we demonstrate how these biases ease the learning problem.

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