The rise of generative AI (GenAI) has impacted many aspects of human life. As these systems become embedded in everyday practices, understanding public trust in them also becomes essential for responsible adoption and governance. Prior work on trust in AI has largely drawn from psychology and human-computer interaction, but there is a lack of computational, large-scale, and longitudinal approaches to measuring trust and distrust in GenAI and large language models (LLMs). This paper presents the first computational study of Trust and Distrust in GenAI, using a multi-year Reddit dataset (2022--2025) spanning 39 subreddits and 197,618 posts. Crowd-sourced annotations of a representative sample were combined with classification models to scale analysis. We find that Trust and Distrust are nearly balanced over time, with shifts around major model releases. Technical performance and usability dominate as dimensions, while personal experience is the most frequent reason shaping attitudes. Distinct patterns also emerge across trustors (e.g., experts, ethicists, general users). Our results provide a methodological framework for large-scale Trust analysis and insights into evolving public perceptions of GenAI.

Reinforcement Learning frameworks, particularly those utilizing human annotations, have become an increasingly popular method for preference fine-tuning, where the outputs of a language model are tuned to match a certain set of behavioral policies or guidelines. Reinforcement Learning through Human Feedback (RLHF) is perhaps the most popular implementation of such a framework, particularly for aligning LMs toward safety and human intent. However, the internal workings of how such alignment is achieved remain largely opaque. In this work, we systematically analyze preference optimization for language model alignment by applying layer-wide causal patching between a base model and its tuned counterpart across human preference pairs. We implement our methodology on \textit{Llama-3.2-1B}, and find that alignment is spatially localized: mid-layer activations encode a distinct subspace that causally determines reward-consistent behavior, while early and late layers remain largely unaffected. Utilizing LASSO regression, we also find that only a small number of layers possess non-zero coefficients linking activation distances to reward gains. Overall, we show that, at least for some language models, alignment from human-based, preferential tuning is a directional, low rank process, rather than diffuse and parameteric.

Scientific literature is increasingly siloed by complex language, static disciplinary structures, and potentially sparse keyword systems, making it cumbersome to capture the dynamic nature of modern science. This study addresses these challenges by introducing an adaptable large language model (LLM)-driven framework to quantify thematic trends and map the evolving landscape of scientific knowledge. The approach is demonstrated over a 20-year collection of more than 1,500 engineering articles published by the Proceedings of the National Academy of Sciences (PNAS), marked for their breadth and depth of research focus. A two-stage classification pipeline first establishes a primary thematic category for each article based on its abstract. The subsequent phase performs a full-text analysis to assign secondary classifications, revealing latent, cross-topic connections across the corpus. Traditional natural language processing (NLP) methods, such as Bag-of-Words (BoW) and Term Frequency-Inverse Document Frequency (TF-IDF), confirm the resulting topical structure and also suggest that standalone word-frequency analyses may be insufficient for mapping fields with high diversity. Finally, a disjoint graph representation between the primary and secondary classifications reveals implicit connections between themes that may be less apparent when analyzing abstracts or keywords alone. The findings show that the approach independently recovers much of the journal's editorially embedded structure without prior knowledge of its existing dual-classification schema (e.g., biological studies also classified as engineering). This framework offers a powerful tool for detecting potential thematic trends and providing a high-level overview of scientific progress.

Creating high-quality clinical Chains-of-Thought (CoTs) is crucial for explainable medical Artificial Intelligence (AI) while constrained by data scarcity. Although Large Language Models (LLMs) can synthesize medical data, their clinical reliability remains unverified. This study evaluates the reliability of LLM-generated CoTs and investigates prompting strategies to enhance their quality. In a blinded comparative study, senior clinicians in Assisted Reproductive Technology (ART) evaluated CoTs generated via three distinct strategies: Zero-shot, Random Few-shot (using shallow examples), and Selective Few-shot (using diverse, high-quality examples). These expert ratings were compared against evaluations from a state-of-the-art AI model (GPT-4o). The Selective Few-shot strategy significantly outperformed other strategies across all human evaluation metrics (p < .001). Critically, the Random Few-shot strategy offered no significant improvement over the Zero-shot baseline, demonstrating that low-quality examples are as ineffective as no examples. The success of the Selective strategy is attributed to two principles: "Gold-Standard Depth" (reasoning quality) and "Representative Diversity" (generalization). Notably, the AI evaluator failed to discern these critical performance differences. The clinical reliability of synthetic CoTs is dictated by strategic prompt curation, not the mere presence of examples. We propose a "Dual Principles" framework as a foundational methodology to generate trustworthy data at scale. This work offers a validated solution to the data bottleneck and confirms the indispensable role of human expertise in evaluating high-stakes clinical AI.

This study quantifies how prompting strategies interact with large language models (LLMs) to automate the screening stage of systematic literature reviews (SLRs). We evaluate six LLMs (GPT-4o, GPT-4o-mini, DeepSeek-Chat-V3, Gemini-2.5-Flash, Claude-3.5-Haiku, Llama-4-Maverick) under five prompt types (zero-shot, few-shot, chain-of-thought (CoT), CoT-few-shot, self-reflection) across relevance classification and six Level-2 tasks, using accuracy, precision, recall, and F1. Results show pronounced model-prompt interaction effects: CoT-few-shot yields the most reliable precision-recall balance; zero-shot maximizes recall for high-sensitivity passes; and self-reflection underperforms due to over-inclusivity and instability across models. GPT-4o and DeepSeek provide robust overall performance, while GPT-4o-mini performs competitively at a substantially lower dollar cost. A cost-performance analysis for relevance classification (per 1,000 abstracts) reveals large absolute differences among model-prompt pairings; GPT-4o-mini remains low-cost across prompts, and structured prompts (CoT/CoT-few-shot) on GPT-4o-mini offer attractive F1 at a small incremental cost. We recommend a staged workflow that (1) deploys low-cost models with structured prompts for first-pass screening and (2) escalates only borderline cases to higher-capacity models. These findings highlight LLMs' uneven but promising potential to automate literature screening. By systematically analyzing prompt-model interactions, we provide a comparative benchmark and practical guidance for task-adaptive LLM deployment.

Abstract--The 2022 World Mental Health Report calls for global mental health care reform, amid rising prevalence of issues like anxiety and depression that affect nearly one billion people worldwide. Traditional in-person therapy fails to meet this demand, and the situation is worsened by stigma. While general-purpose large language models (LLMs) offer efficiency for AI-driven mental health solutions, they underperform because they lack specialized fine-tuning. Existing LLM-based mental health chatbots can engage in empathetic conversations, but they overlook real-time user mental state assessment which is critical for professional counseling. This paper proposes MoPHES, a framework that integrates mental state evaluation, conversational support, and professional treatment recommendations. The agent developed under this framework uses two fine-tuned MiniCPM4-0.5B LLMs: one is fine-tuned on mental health conditions datasets to assess users' mental states and predict the severity of anxiety and depression; the other is fine-tuned on multi-turn dialogues to handle conversations with users. By leveraging insights into users' mental states, our agent provides more tailored support and professional treatment recommendations. Both models are also deployed directly on mobile devices to enhance user convenience and protect user privacy. Additionally, to evaluate the performance of MoPHES with other LLMs, we develop a benchmark for the automatic evaluation of mental state prediction and multi-turn counseling dialogues, which includes comprehensive evaluation metrics, datasets, and methods. ENT AL health issues are emerging as an increasingly severe threat to global public health, with their prevalence rising annually.

Credential stuffing attacks have caused significant harm to online users who frequently reuse passwords across multiple websites. While prior research has attempted to detect users with reused passwords or identify malicious login attempts, existing methods often compromise usability by restricting password creation or website access, and their reliance on complex account-sharing mechanisms hinders real-world deployment. To address these limitations, we propose PassREfinder-FL, a novel framework that predicts credential stuffing risks across websites. We introduce the concept of password reuse relations -- defined as the likelihood of users reusing passwords between websites -- and represent them as edges in a website graph. Using graph neural networks (GNNs), we perform a link prediction task to assess credential reuse risk between sites. Our approach scales to a large number of arbitrary websites by incorporating public website information and linking newly observed websites as nodes in the graph. To preserve user privacy, we extend PassREfinder-FL with a federated learning (FL) approach that eliminates the need to share user sensitive information across administrators. Evaluation on a real-world dataset of 360 million breached accounts from 22,378 websites shows that PassREfinder-FL achieves an F1-score of 0.9153 in the FL setting. We further validate that our FL-based GNN achieves a 4-11% performance improvement over other state-of-the-art GNN models through an ablation study. Finally, we demonstrate that the predicted results can be used to quantify password reuse likelihood as actionable risk scores.

While Artificial Intelligence (AI) shows promise in healthcare applications, existing conversational systems often falter in complex and sensitive medical domains such as Sexual and Reproductive Health (SRH). These systems frequently struggle with hallucination and lack the specialized knowledge required, particularly for sensitive SRH topics. Furthermore, current AI approaches in healthcare tend to prioritize diagnostic capabilities over comprehensive patient care and education. Addressing these gaps, this work at the UNC School of Nursing introduces SARHAchat, a proof-of-concept Large Language Model (LLM)- based chatbot. SARHAchat is designed as a reliable, user-centered system integrating medical expertise with empathetic communication to enhance SRH care delivery. Our evaluation demonstrates SARHAchat's ability to provide accurate and contextually appropriate contraceptive counseling while maintaining a natural conversational flow. The demo is available at https://sarhachat.com/.

Emergency departments worldwide face rising patient volumes, workforce shortages, and variability in triage decisions that threaten the delivery of timely and accurate care. Current triage methods rely primarily on vital signs, routine laboratory values, and clinicians' judgment, which, while effective, often miss emerging biological signals that could improve risk prediction for infection typing or antibiotic administration in acute conditions. To address this challenge, we introduce TriAgent, a large language model (LLM)-based multi-agent framework that couples automated biomarker discovery with deep research for literature-grounded validation and novelty assessment. TriAgent employs a supervisor research agent to generate research topics and delegate targeted queries to specialized sub-agents for evidence retrieval from various data sources. Findings are synthesized to classify biomarkers as either grounded in existing knowledge or flagged as novel candidates, offering transparent justification and highlighting unexplored pathways in acute care risk stratification. Unlike prior frameworks limited to existing routine clinical biomarkers, TriAgent aims to deliver an end-to-end framework from data analysis to literature grounding to improve transparency, explainability and expand the frontier of potentially actionable clinical biomarkers. Given a user's clinical query and quantitative triage data, TriAgent achieved a topic adherence F1 score of 55.7 +/- 5.0%, surpassing the CoT-ReAct agent by over 10%, and a faithfulness score of 0.42 +/- 0.39, exceeding all baselines by more than 50%. Across experiments, TriAgent consistently outperformed state-of-the-art LLM-based agentic frameworks in biomarker justification and literature-grounded novelty assessment. We share our repo: https://github.com/CellFace/TriAgent.

Current Large Language Model (LLM) agents show strong performance in tool use, but lack the crucial capability to systematically learn from their own experiences. While existing frameworks mainly focus on mitigating external knowledge gaps, they fail to address a more fundamental limitation: the inability to itera-tively refine problem-solving strategies. In this work, we introduce EvolveR, a framework designed to enable agent to self-improve through a complete, closed-loop experience lifecycle. This lifecycle comprises two key stages: (1) Offline Self-Distillation, where the agent's interaction trajectories are synthesized into a structured repository of abstract, reusable strategic principles; (2) Online Interaction, where the agent interacts with tasks and actively retrieves distilled principles to guide its decision-making, accumulating a diverse set of behavioral trajectories. This loop employs a policy reinforcement mechanism to it-eratively update the agent based on its performance. We demonstrate the effectiveness of EvolveR on complex multi-hop question-answering benchmarks, where it achieves superior performance over strong agentic baselines. Our work presents a comprehensive blueprint for agents that learn not only from external data but also from the consequences of their own actions, paving the way for more autonomous and continuously improving systems. Code is available at https://github.com/Edaizi/EvolveR. Large Language Models (LLMs) have driven the development of autonomous agents capable of solving diverse tasks through advanced reasoning and tool use [1-3]. However, a significant limitation emerges when these agents engage in sequential tasks: each interaction is treated independently. They approach tasks as isolated episodes, suffering from operational amnesia and failing to learn from past successes or avoid prior mistakes[4].