Manual tissue extraction from potato tubers for molecular pathogen detection is highly laborious. This study presents a machine-vision-guided, dual-arm coordinated inline robotic system integrating tuber grasping and tissue sampling mechanisms. Tubers are transported on a conveyor that halts when a YOLOv11-based vision system detects a tuber within the workspace of a one-prismatic-degree-of-freedom (P-DoF) robotic arm. This arm, equipped with a gripping end-effector, secures and positions the tuber for sampling. The second arm, a 3-P-DoF Cartesian manipulator with a biopsy punch-based end-effector, then performs tissue extraction guided by a YOLOv10-based vision system that identifies the sampling sites on the tuber such as eyes or stolon scars. The sampling involves four stages: insertion of the punch into the tuber, punch rotation for tissue detachment, biopsy punch retraction, and deposition of the tissue core onto a collection site. The system achieved an average positional error of 1.84 mm along the tuber surface and a depth deviation of 1.79 mm from a 7.00 mm target. The success rate for core extraction and deposition was 81.5%, with an average sampling cycle of 10.4 seconds. The total cost of the system components was under $1,900, demonstrating the system's potential as a cost-effective alternative to labor-intensive manual tissue sampling. Future work will focus on optimizing for multi-site sampling from a single tuber and validation in commercial settings.

The vigor of potato plants, defined as the canopy area at the end of the exponential growth stage, depends on the origin and physiological state of the seed tuber. Experiments carried out with six potato varieties in three test fields over three years show that there is a 73%-90% correlation in the vigor of the plants from the same seedlot grown in different test fields. However, these correlations are not always observed on the level of individual varieties and vanish or become negative when the seed tubers and young plants experience environmental stress. A comprehensive study of the association between the vigor and the seed tuber biochemistry has revealed that, while 50%-70% of the variation in the plant vigor is explained by the tuber data, the vigor is dominated by the potato genotype. Analysis of individual predictors, such as the abundance of a particular metabolite, indicates that the vigor enhancing properties of the seed tubers differ between genotypes. Variety-specific models show that, for some varieties, up to 30% of the vigor variation within the variety is explained by and can be predicted from the tuber biochemistry, whereas, for other varieties, the association between the tuber composition and the vigor is much weaker.