In cell culture bioprocessing, real-time batch process monitoring (BPM) refers to the continuous tracking and analysis of key process variables such as viable cell density, nutrient levels, metabolite concentrations, and product titer throughout the duration of a batch run. This enables early detection of deviations and supports timely control actions to ensure optimal cell growth and product quality. BPM plays a critical role in ensuring the quality and regulatory compliance of biopharmaceutical manufacturing processes. However, the development of accurate soft sensors for BPM is hindered by key challenges, including limited historical data, infrequent feedback, heterogeneous process conditions, and high-dimensional sensory inputs. This study presents a comprehensive benchmarking analysis of machine learning (ML) methods designed to address these challenges, with a focus on learning from historical data with limited volume and relevance in the context of bioprocess monitoring. We evaluate multiple ML approaches including feature dimensionality reduction, online learning, and just-in-time learning across three datasets, one in silico dataset and two real-world experimental datasets. Our findings highlight the importance of training strategies in handling limited data and feedback, with batch learning proving effective in homogeneous settings, while just-in-time learning and online learning demonstrate superior adaptability in cold-start scenarios. Additionally, we identify key meta-features, such as feed media composition and process control strategies, that significantly impact model transferability. The results also suggest that integrating Raman-based predictions with lagged offline measurements enhances monitoring accuracy, offering a promising direction for future bioprocess soft sensor development.

This study explores the deployment of three machine learning (ML) approaches for real-time prediction of glucose, lactate, and ammonium concentrations in cell culture processes, using Raman spectroscopy as input features. The research addresses challenges associated with limited data availability and process variability, providing a comparative analysis of pretrained models, just-in-time learning (JITL), and online learning algorithms. Two industrial case studies are presented to evaluate the impact of varying bioprocess conditions on model performance. The findings highlight the specific conditions under which pretrained models demonstrate superior predictive accuracy and identify scenarios where JITL or online learning approaches are more effective for adaptive process monitoring. This study also highlights the critical importance of updating the deployed models/agents with the latest offline analytical measurements during bioreactor operations to maintain the model performance against the changes in cell growth behaviours and operating conditions throughout the bioreactor run. Additionally, the study confirms the usefulness of a simple mixture-of-experts framework in achieving enhanced accuracy and robustness for real-time predictions of metabolite concentrations based on Raman spectral data. These insights contribute to the development of robust strategies for the efficient deployment of ML models in dynamic and changing biomanufacturing environments.

Welcome to our monthly digest, where you can catch up with any AIhub stories you may have missed, peruse the latest news, recap recent events, and more. This month, we learn about drug and material design using generative models and Bayesian optimization, find out about a system for real-time monitoring for healthcare data, and explore domain-specific distribution shifts in volunteer-collected biodiversity datasets. Ananya Joshi recently completed her PhD, where she developed a system that experts have used for the past two years to identify respiratory outbreaks (like COVID-19) in large-scale healthcare streams across the United States. In this interview, she tells us more about this project, how healthcare applications inspire basic AI research, and her future plans. Onur Boyar is a PhD student at Nagoya university, working on generative models and Bayesian methods for materials and drug design.

--Digital twins (DTs) help improve real-time monitoring and decision-making in water distribution systems. However, their connectivity makes them easy targets for cyberattacks such as scanning, denial-of-service (DoS), and unauthorized access. Small and medium-sized enterprises (SMEs) that manage these systems often do not have enough budget or staff to build strong cybersecurity teams. T o solve this problem, we present a Virtual Cybersecurity Department (VCD), an affordable and automated framework designed for SMEs. The VCD uses open-source tools like Zabbix for real-time monitoring, Suricata for network intrusion detection, Fail2Ban to block repeated login attempts, and simple firewall settings. T o improve threat detection, we also add a machine-learning-based IDS trained on the OD-IDS2022 dataset using an improved ensemble model. Our solution gives SMEs a practical and efficient way to secure water systems using low-cost and easy-to-manage tools.

Effective risk management solutions become absolutely crucial when financial markets embrace distributed technology and decentralized financing (DeFi). This study offers a thorough survey and comparative analysis of the integration of artificial intelligence (AI) in risk management for distributed arbitrage systems. We examine several modern caching techniques namely in memory caching, distributed caching, and proxy caching and their functions in enhancing performance in decentralized settings. Through literature review we examine the utilization of AI techniques for alleviating risks related to market volatility, liquidity challenges, operational failures, regulatory compliance, and security threats. This comparison research evaluates various case studies from prominent DeFi technologies, emphasizing critical performance metrics like latency reduction, load balancing, and system resilience. Additionally, we examine the problems and trade offs associated with these technologies, emphasizing their effects on consistency, scalability, and fault tolerance. By meticulously analyzing real world applications, specifically centering on the Aave platform as our principal case study, we illustrate how the purposeful amalgamation of AI with contemporary caching methodologies has revolutionized risk management in distributed arbitrage systems.

As the global population grows and climate change intensifies, sustainable food production is critical. Marine aquaculture offers a viable solution, providing a sustainable protein source. However, the industry's expansion requires novel technologies for remote management and autonomous operations. Digital twin technology can advance the aquaculture industry, but its adoption has been limited. Fish net cages, which are flexible floating structures, are critical yet vulnerable components of aquaculture farms. Exposed to harsh and dynamic marine environments, the cages experience significant loads and risk damage, leading to fish escapes, environmental impacts, and financial losses. We propose a multifidelity surrogate modeling framework for integration into a digital twin for real-time monitoring of aquaculture net cage structural dynamics under stochastic marine conditions. Central to this framework is the nonlinear autoregressive Gaussian process method, which learns complex, nonlinear cross-correlations between models of varying fidelity. It combines low-fidelity simulation data with a small set of high-fidelity field sensor measurements, which offer the real dynamics but are costly and spatially sparse. Validated at the SINTEF ACE fish farm in Norway, our digital twin receives online metocean data and accurately predicts net cage displacements and mooring line loads, aligning closely with field measurements. The proposed framework is beneficial where application-specific data are scarce, offering rapid predictions and real-time system representation. The developed digital twin prevents potential damages by assessing structural integrity and facilitates remote operations with unmanned underwater vehicles. Our work also compares GP and GCNs for predicting net cage deformation, highlighting the latter's effectiveness in complex structural applications.

We consider two ubiq- uitous processes: an industrial dryer and a level tank. For these appli- cations, we compared three particle filtering variants: standard parti- cle filtering, Rao-Blackwellised particle filtering and a version of Rao- Blackwellised particle filtering that does one-step look-ahead to select good sampling regions. We show that the overhead of the extra process- ing per particle of the more sophisticated methods is more than compen- sated by the decrease in error and variance.

The world is in a climate crisis. With average global temperatures increasing every year, the threat of seasonal forest fires is becoming increasingly worse. In places like the Pacific Northwest, wildfire season causes extensive damage to woodlands, rural communities, and townships, destroying farmlands and infrastructure and forcing hundreds of thousands of residents to flee their homes. These fires also lead to terrible air quality in cities located hundreds (or even thousands) of miles away. For instance, in September of 2022, the city of Vancouver (British Columbia) was ranked as having the worst air quality in the world - per the Air Quality Index (AQI).

If you read news, follow research articles, and discuss the future, then you would have already read about how the healthcare industry is in dire need of AI. If I were to pick the prominent industries which can drive sustainable growth healthcare would be on the top. Perhaps it is the reason that majority of new research now includes terms "renewable, sustainable, and eco friendly". Humans have realized that growth at the risk of environmental deterioration, and deteriorating human health is no growth at all. There is another parallel industry that is being explored by the inventors and it is "artificial intelligence".

RAILWAY AGE, JULY 2021 ISSUE: Reliable, real-time monitoring of in-service railcar components will enhance the potential for maintenance planning. Through the Association of American Railroads (AAR) Strategic Research Initiatives (SRI) program, Transportation Technology Center, Inc. (TTCI) has been assisting suppliers and other stakeholders in the development of machine vision technologies and related algorithms for evaluating railcar components and conditions. To enhance safety and reduce worker exposure to yard risk, North American railroads have begun to install machine vision inspection systems in revenue service. Using commercially available deep learning system platforms, TTCI researchers developed and demonstrated three convolutional neural network-based applications for analyzing visual images. A convolutional neural network is a type of artificial neural network that uses machine learning algorithms to analyze digital images. Convolutional neural networks are more powerful and effective than traditional artificial neural networks at recognizing, interpreting, and categorizing large, unstructured data sets; particularly those comprised of visual imagery.