-- In this paper, we introduce Haptic-Informed ACT, an advanced robotic system for pseudo oocyte manipulation, integrating multimodal information and Action Chunking with Transformers (ACT). Traditional automation methods for oocyte transfer rely heavily on visual perception, often requiring human supervision due to biological variability and environmental disturbances. Haptic-Informed ACT enhances ACT by incorporating haptic feedback, enabling real-time grasp failure detection and adaptive correction. Additionally, we introduce a 3D-printed TPU soft gripper to facilitate delicate manipulations. Experimental results demonstrate that Haptic-Informed ACT improves the task success rate, robustness, and adaptability compared to conventional ACT, particularly in dynamic environments. Manipulation of cells is the basis for many applications in biological and biomedical engineering.

Artificial intelligence (AI) has emerged as a powerful tool to enhance decision-making and optimize treatment protocols in in vitro fertilization (IVF). In particular, AI shows significant promise in supporting decision-making during the ovarian stimulation phase of the IVF process. This review evaluates studies focused on the applications of AI combined with medical imaging in ovarian stimulation, examining methodologies, outcomes, and current limitations. Our analysis of 13 studies on this topic reveals that, reveal that while AI algorithms demonstrated notable potential in predicting optimal hormonal dosages, trigger timing, and oocyte retrieval outcomes, the medical imaging data utilized predominantly came from two-dimensional (2D) ultrasound which mainly involved basic quantifications, such as follicle size and number, with limited use of direct feature extraction or advanced image analysis techniques. This points to an underexplored opportunity where advanced image analysis approaches, such as deep learning, and more diverse imaging modalities, like three-dimensional (3D) ultrasound, could unlock deeper insights. Additionally, the lack of explainable AI (XAI) in most studies raises concerns about the transparency and traceability of AI-driven decisions - key factors for clinical adoption and trust. Furthermore, many studies relied on single-center designs and small datasets, which limit the generalizability of their findings. This review highlights the need for integrating advanced imaging analysis techniques with explainable AI methodologies, as well as the importance of leveraging multicenter collaborations and larger datasets. Addressing these gaps has the potential to enhance ovarian stimulation management, paving the way for efficient, personalized, and data-driven treatment pathways that improve IVF outcomes.

The investigation of dynamical processes on networks has been one focus for the study of contagion processes. It has been demonstrated that contagions can be used to obtain information about the embedding of nodes in a Euclidean space. Specifically, one can use the activation times of threshold contagions to construct contagion maps as a manifold-learning approach. One drawback of contagion maps is their high computational cost. Here, we demonstrate that a truncation of the threshold contagions may considerably speed up the construction of contagion maps. Finally, we show that contagion maps may be used to find an insightful low-dimensional embedding for single-cell RNA-sequencing data in the form of cell-similarity networks and so reveal biological manifolds. Overall, our work makes the use of contagion maps as manifold-learning approaches on empirical network data more viable.

Meiotic maturation is a crucial step of oocyte formation allowing its potential fertilization and embryo development. Elucidating this process is important both for fundamental research and assisted reproductive technology. Few computational tools based on non-invasive measurements are however available to characterize oocyte meiotic maturation. Here, we develop a computational framework to phenotype oocytes based on images acquired in transmitted light. We trained neural networks to segment the contour of oocytes and their zona pellucida using oocytes from diverse species. We defined a comprehensive set of morphological features to describe an oocyte. These steps are implemented in an open-source Fiji plugin. We present a feature based machine learning pipeline to recognize oocyte populations and determine their morphological differences. We first demonstrate its potential to screen oocyte from different strains and automatically identify their morphological characteristics. Its second application is to predict and characterize the maturation potential of oocytes. We identify the texture of the zona pellucida and the cytoplasmic particles size as features to assess mouse oocyte maturation potential and tested whether these features were applicable to human oocyte's developmental potential.