Marmoset, a highly vocalized primate, has become a popular animal model for studying social-communicative behavior and its underlying mechanism comparing with human infant linguistic developments. In the study of vocal communication, it is vital to know the caller identities, call contents, and vocal exchanges. Previous work of a CNN has achieved a joint model for call segmentation, classification, and caller identification for marmoset vocalizations. However, the CNN has limitations in modeling long-range acoustic patterns; the Transformer architecture that has been shown to outperform CNNs, utilizes the self-attention mechanism that efficiently segregates information parallelly over long distances and captures the global structure of marmoset vocalization. We propose using the Transformer to jointly segment and classify the marmoset calls and identify the callers for each vocalization.

Marmosets use unique calls for other monkeys in their family groups, similar to how humans call each other by name. They are the first non-human primates known to do so. This discovery shows that communication in marmosets is more complex than previously thought, and it could help teach us more about how human language evolved. "Up till quite recently, people thought that human language is a singularity phenomenon that popped out of nothing," says David Omer at The Hebrew University of Jerusalem. "We're starting to see evidence that this is not the case."

Marmoset monkeys encode vital information in their calls and serve as a surrogate model for neuro-biologists to understand the evolutionary origins of human vocal communication. Traditionally analyzed with signal processing-based features, recent approaches have utilized self-supervised models pre-trained on human speech for feature extraction, capitalizing on their ability to learn a signal's intrinsic structure independently of its acoustic domain. However, the utility of such foundation models remains unclear for marmoset call analysis in terms of multi-class classification, bandwidth, and pre-training domain. This study assesses feature representations derived from speech and general audio domains, across pre-training bandwidths of 4, 8, and 16 kHz for marmoset call-type and caller classification tasks. Results show that models with higher bandwidth improve performance, and pre-training on speech or general audio yields comparable results, improving over a spectral baseline.

Chronic gastrointestinal (GI) diseases are the most common diseases in captive common marmosets (Callithrix jacchus). Despite standardized housing, diet and husbandry, a recently described gastrointestinal syndrome characterized by duodenal ulcers and strictures was observed in a subset of marmosets sourced from the New England Primate Research Center. As changes in the gut microbiome have been associated with GI diseases, the gut microbiome of 52 healthy, non-stricture marmosets (153 samples) were compared to the gut microbiome of 21 captive marmosets diagnosed with a duodenal ulcer/stricture (57 samples). No significant changes were observed using alpha diversity metrics, and while the community structure was significantly different when comparing beta diversity between healthy and stricture cases, the results were inconclusive due to differences observed in the dispersion of both datasets. Differences in the abundance of individual taxa using ANCOM, as stricture-associated dysbiosis was characterized by Anaerobiospirillum loss and Clostridium perfringens increases. To identify microbial and serum biomarkers that could help classify stricture cases, we developed models using machine learning algorithms (random forest, classification and regression trees, support vector machines and k-nearest neighbors) to classify microbiome, serum chemistry or complete blood count (CBC) data. Random forest (RF) models were the most accurate models and correctly classified strictures using either 9 ASVs (amplicon sequence variants), 4 serum chemistry tests or 6 CBC tests. Based on the RF model and ANCOM results, C. perfringens was identified as a potential causative agent associated with the development of strictures. Clostridium perfringens was also isolated by microbiological culture in 4 of 9 duodenum samples from marmosets with histologically confirmed strictures. Due to the enrichment of C. perfringens in situ, we analyzed frozen duodenal tissues using both 16S microbiome profiling and RNAseq. Microbiome analysis of the duodenal tissues of 29 marmosets from the MIT colony confirmed an increased abundance of Clostridium in stricture cases. Comparison of the duodenal gene expression from stricture and non-stricture marmosets found enrichment of genes associated with intestinal absorption, and lipid metabolism, localization, and transport in stricture cases. Using machine learning, we identified increased abundance of C. perfringens, as a potential causative agent of GI disease and intestinal strictures in marmosets.

In this work we propose a new biophysical computational model of brain regions relevant to Parkinson's Disease based on local field potential data collected from the brain of marmoset monkeys. Parkinson's disease is a neurodegenerative disorder, linked to the death of dopaminergic neurons at the substantia nigra pars compacta, which affects the normal dynamics of the basal ganglia-thalamus-cortex neuronal circuit of the brain. Although there are multiple mechanisms underlying the disease, a complete description of those mechanisms and molecular pathogenesis are still missing, and there is still no cure. To address this gap, computational models that resemble neurobiological aspects found in animal models have been proposed. In our model, we performed a data-driven approach in which a set of biologically constrained parameters is optimised using differential evolution. Evolved models successfully resembled single-neuron mean firing rates and spectral signatures of local field potentials from healthy and parkinsonian marmoset brain data. As far as we are concerned, this is the first computational model of Parkinson's Disease based on simultaneous electrophysiological recordings from seven brain regions of Marmoset monkeys. Results show that the proposed model could facilitate the investigation of the mechanisms of PD and support the development of techniques that can indicate new therapies. It could also be applied to other computational neuroscience problems in which biological data could be used to fit multi-scale models of brain circuits.

An insistent pattern has quietly taken hold in my household. I will order some consumer product online. I will open the package, extract the thing from its protective wrappings, and retrieve the instruction manual. I will examine the product briefly, then begin to read the instruction manual. And then I will go to YouTube.

Despite years of research, the brain still contains broad areas of unchartered territory. A team of scientists, led by neuroscientists from Cold Spring Harbor Laboratory and University of Sydney, recently found new evidence revising the traditional view of the primate brain's visual system organization using data from marmosets. This remapping of the brain could serve as a future reference for understanding how the highly complex visual system works, and potentially influence the design of artificial neural networks for machine vision. In the quest of the whole-brain connectivity in marmosets, the team found that parts of the primate visual system may work differently than previously thought. Mapping out how distinct types of cells connect can help researchers understand how groups of cells play in concert to relay and process sensory information from the outside environment to the brain.

An insistent pattern has quietly taken hold in my household. I will order some consumer product online. I will open the package, extract the thing from its protective wrappings, and retrieve the instruction manual. I will examine the product briefly, then begin to read the instruction manual. And then I will go to YouTube.