The Frozen Section (FS) technique is a rapid and efficient method, taking only 15-30 minutes to prepare slides for pathologists' evaluation during surgery, enabling immediate decisions on further surgical interventions. However, FS process often introduces artifacts and distortions like folds and ice-crystal effects. In contrast, these artifacts and distortions are absent in the higher-quality formalin-fixed paraffin-embedded (FFPE) slides, which require 2-3 days to prepare. While Generative Adversarial Network (GAN)-based methods have been used to translate FS to FFPE images (F2F), they may leave morphological inaccuracies with remaining FS artifacts or introduce new artifacts, reducing the quality of these translations for clinical assessments. In this study, we benchmark recent generative models, focusing on GANs and Latent Diffusion Models (LDMs), to overcome these limitations. We introduce a novel approach that combines LDMs with Histopathology Pre-Trained Embeddings to enhance restoration of FS images. Our framework leverages LDMs conditioned by both text and pre-trained embeddings to learn meaningful features of FS and FFPE histopathology images. Through diffusion and denoising techniques, our approach not only preserves essential diagnostic attributes like color staining and tissue morphology but also proposes an embedding translation mechanism to better predict the targeted FFPE representation of input FS images. As a result, this work achieves a significant improvement in classification performance, with the Area Under the Curve rising from 81.99% to 94.64%, accompanied by an advantageous CaseFD. This work establishes a new benchmark for FS to FFPE image translation quality, promising enhanced reliability and accuracy in histopathology FS image analysis. Our work is available at https://minhmanho.github.io/f2f_ldm/.

With the advent of digital scanners and deep learning, diagnostic operations may move from a microscope to a desktop. Hematoxylin and Eosin (H&E) staining is one of the most frequently used stains for disease analysis, diagnosis, and grading, but pathologists do need different immunohistochemical (IHC) stains to analyze specific structures or cells. Obtaining all of these stains (H&E and different IHCs) on a single specimen is a tedious and time-consuming task. Consequently, virtual staining has emerged as an essential research direction. Here, we propose a novel generative model, Structural Cycle-GAN (SC-GAN), for synthesizing IHC stains from H&E images, and vice versa. Our method expressly incorporates structural information in the form of edges (in addition to color data) and employs attention modules exclusively in the decoder of the proposed generator model. This integration enhances feature localization and preserves contextual information during the generation process. In addition, a structural loss is incorporated to ensure accurate structure alignment between the generated and input markers. To demonstrate the efficacy of the proposed model, experiments are conducted with two IHC markers emphasizing distinct structures of glands in the colon: the nucleus of epithelial cells (CDX2) and the cytoplasm (CK818). Quantitative metrics such as FID and SSIM are frequently used for the analysis of generative models, but they do not correlate explicitly with higher-quality virtual staining results. Therefore, we propose two new quantitative metrics that correlate directly with the virtual staining specificity of IHC markers.

Automatic transcription of scene understanding in images and videos is a step towards artificial general intelligence. Image captioning is a nomenclature for describing meaningful information in an image using computer vision techniques. Automated image captioning techniques utilize encoder and decoder architecture, where the encoder extracts features from an image and the decoder generates a transcript. In this work, we investigate two unexplored ideas for image captioning using transformers: First, we demonstrate the enforcement of using objects' relevance in the surrounding environment. Second, learning an explicit association between labels and language constructs. We propose label-attention Transformer with geometrically coherent objects (LATGeO). The proposed technique acquires a proposal of geometrically coherent objects using a deep neural network (DNN) and generates captions by investigating their relationships using a label-attention module. Object coherence is defined using the localized ratio of the geometrical properties of the proposals. The label-attention module associates the extracted objects classes to the available dictionary using self-attention layers. The experimentation results show that objects' relevance in surroundings and binding of their visual feature with their geometrically localized ratios combined with its associated labels help in defining meaningful captions. The proposed framework is tested on the MSCOCO dataset, and a thorough evaluation resulting in overall better quantitative scores pronounces its superiority.

Real-time image captioning, along with adequate precision, is the main challenge of this research field. The present work, Multiple Transformers for Self-Attention Mechanism (MTSM), utilizes multiple transformers to address these problems. The proposed algorithm, MTSM, acquires region proposals using a transformer detector (DETR). Consequently, MTSM achieves the self-attention mechanism by transferring these region proposals and their visual and geometrical features through another transformer and learns the objects' local and global interconnections. The qualitative and quantitative results of the proposed algorithm, MTSM, are shown on the MSCOCO dataset.