The rise of artificial intelligence (AI) and the aggravating biodiversity crisis have resulted in a research area where AI-based computational methods are being developed to act as allies in conservation, and the sustainable use and management of natural resources. While important general guidelines have been established globally regarding the opportunities and challenges that this interdisciplinary research offers, it is essential to generate local reflections from the specific contexts and realities of each region. Hence, this document aims to analyze the scope of this research area from a perspective focused on Colombia and the Neotropics. In this paper, we summarize the main experiences and debates that took place at the Humboldt Institute between 2023 and 2024 in Colombia. To illustrate the variety of promising opportunities, we present current uses such as automatic species identification from images and recordings, species modeling, and in silico bioprospecting, among others. From the experiences described above, we highlight limitations, challenges, and opportunities for in order to successfully implementate AI in conservation efforts and sustainable management of biological resources in the Neotropics. The result aims to be a guide for researchers, decision makers, and biodiversity managers, facilitating the understanding of how artificial intelligence can be effectively integrated into conservation and sustainable use strategies. Furthermore, it also seeks to open a space for dialogue on the development of policies that promote the responsible and ethical adoption of AI in local contexts, ensuring that its benefits are harnessed without compromising biodiversity or the cultural and ecosystemic values inherent in Colombia and the Neotropics.

Global change is predicted to induce shifts in anuran acoustic behavior, which can be studied through passive acoustic monitoring (PAM). Understanding changes in calling behavior requires the identification of anuran species, which is challenging due to the particular characteristics of neotropical soundscapes. In this paper, we introduce a large-scale multi-species dataset of anuran amphibians calls recorded by PAM, that comprises 27 hours of expert annotations for 42 different species from two Brazilian biomes. We provide open access to the dataset, including the raw recordings, experimental setup code, and a benchmark with a baseline model of the fine-grained categorization problem. Additionally, we highlight the challenges of the dataset to encourage machine learning researchers to solve the problem of anuran call identification towards conservation policy.