Recent advances in neural interfacing have enabled significant improvements in human-computer interaction, rehabilitation, and neuromuscular diagnostics. Motor unit (MU) decomposition from surface electromyography (sEMG) is a key technique for extracting neural drive information, but traditional blind source separation (BSS) methods fail to incorporate biophysical constraints, limiting their accuracy and interpretability. In this work, we introduce a novel Biophysical-Model-Informed Source Separation (BMISS) framework, which integrates anatomically accurate forward EMG models into the decomposition process. By leveraging MRI-based anatomical reconstructions and generative modeling, our approach enables direct inversion of a biophysically accurate forward model to estimate both neural drive and motor neuron properties in an unsupervised manner. Empirical validation in a controlled simulated setting demonstrates that BMISS achieves higher fidelity motor unit estimation while significantly reducing computational cost compared to traditional methods. This framework paves the way for non-invasive, personalized neuromuscular assessments, with potential applications in clinical diagnostics, prosthetic control, and neurorehabilitation.

Laboratory for Intelligent Systems and Informatics, Department of Mechano-Informatics, Graduate School of Information Science and Technology, TheUniversity of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan (Dated: August 21, 2025) Neuronal spikes directly drive muscles and endow animals with agile movements, but applying the spike-based control signals to actuators in artificial sensor-motor systems inevitably causes a collapse of learning. We developed a system that can vary the number of independent synaptic bundles in sensor-to-motor connections. This paper demonstrates the following four findings: (i) Learning collapses once the number of motor neurons or the number of independent synaptic bundles exceeds a critical limit. The functions of spikes remain largely unknown. Identifying the parameter range in which learning systems using spikes can be constructed will make it possible to study the functions of spikes that were previously inaccessible due to the difficulty of learning.

The nociceptive withdrawal reflex (NWR) is a mechanism to mediate interactions and protect the body from damage in a potentially dangerous environment. To better convey warning signals to users of prosthetic arms or autonomous robots and protect them by triggering a proper NWR, it is useful to use a biological representation of temperature information for fast and effective processing. In this work, we present a neuromorphic spiking network for heat-evoked NWR by mimicking the structure and encoding scheme of the reflex arc. The network is trained with the bio-plausible reward modulated spike timing-dependent plasticity learning algorithm. We evaluated the proposed model and three other methods in recent studies that trigger NWR in an experiment with radiant heat. We found that only the neuromorphic model exhibits the spatial summation (SS) effect and temporal summation (TS) effect similar to humans and can encode the reflex strength matching the intensity of the stimulus in the relative spike latency online. The improved bio-plausibility of this neuromorphic model could improve sensory feedback in neural prostheses.

Decoding nervous system activity is a key challenge in neuroscience and neural interfacing. In this study, we propose a novel neural decoding system that enables unprecedented large-scale sampling of muscle activity. Using micro-electrode arrays with more than 100 channels embedded within the forearm muscles, we recorded high-density signals that captured multi-unit motor neuron activity. This extensive sampling was complemented by advanced methods for neural decomposition, analysis, and classification, allowing us to accurately detect and interpret the spiking activity of spinal motor neurons that innervate hand muscles. We evaluated this system in two healthy participants, each implanted with three electromyogram (EMG) micro-electrode arrays (comprising 40 electrodes each) in the forearm. These arrays recorded muscle activity during both single- and multi-digit isometric contractions. For the first time under controlled conditions, we demonstrate that multi-digit tasks elicit unique patterns of motor neuron recruitment specific to each task, rather than employing combinations of recruitment patterns from single-digit tasks. This observation led us to hypothesize that hand tasks could be classified with high precision based on the decoded neural activity. We achieved perfect classification accuracy (100%) across 12 distinct single- and multi-digit tasks, and consistently high accuracy (>96\%) across all conditions and subjects, for up to 16 task classes. These results significantly outperformed conventional EMG classification methods. The exceptional performance of this system paves the way for developing advanced neural interfaces based on invasive high-density EMG technology. This innovation could greatly enhance human-computer interaction and lead to substantial improvements in assistive technologies, offering new possibilities for restoring motor function in clinical applications.

Following limb amputation, neural signals for limb functions persist in the residual peripheral nerves. Targeted muscle reinnervation (TMR) allows to redirected these signals into spare muscles to recover the neural information through electromyography (EMG). However, a significant challenge arises in separating distinct neural commands redirected from the transferred nerves to the muscles. Disentangling overlapping signals from EMG recordings remains complex, as they can contain mixed neural information that complicates limb function interpretation. To address this challenge, Regenerative Peripheral Nerve Interfaces (RPNIs) surgically partition the nerve into individual fascicles that reinnervate specific muscle grafts, isolating distinct neural sources for more precise control and interpretation of EMG signals. We introduce a novel biointerface that combines TMR surgery of polyvalent nerves with a high-density micro-electrode array implanted at a single site within a reinnervated muscle. Instead of surgically identifying distinct nerve fascicles, our approach separates all neural signals that are re-directed into a single muscle, using the high spatio-temporal selectivity of the micro-electrode array and mathematical source separation methods. We recorded EMG signals from four reinnervated muscles while volunteers performed phantom limb tasks. The decomposition of these signals into motor unit activity revealed distinct clusters of motor neurons associated with diverse functional tasks. Notably, our method enabled the extraction of multiple neural commands within a single reinnervated muscle, eliminating the need for surgical nerve division. This approach not only has the potential of enhancing prosthesis control but also uncovers mechanisms of motor neuron synergies following TMR, providing valuable insights into how the central nervous system encodes movement after reinnervation.

This study introduces an artificial neural network (ANN) for image classification task, inspired by the aversive olfactory learning circuits of the nematode Caenorhabditis elegans (C. elegans). Despite the remarkable performance of ANNs in a variety of tasks, they face challenges such as excessive parameterization, high training costs and limited generalization capabilities. C. elegans, with its simple nervous system comprising only 302 neurons, serves as a paradigm in neurobiological research and is capable of complex behaviors including learning. This research identifies key neural circuits associated with aversive olfactory learning in C. elegans through behavioral experiments and high-throughput gene sequencing, translating them into an image classification ANN architecture. Additionally, two other image classification ANNs with distinct architectures were constructed for comparative performance analysis to highlight the advantages of bio-inspired design. The results indicate that the ANN inspired by the aversive olfactory learning circuits of C. elegans achieves higher accuracy, better consistency and faster convergence rates in image classification task, especially when tackling more complex classification challenges. This study not only showcases the potential of bio-inspired design in enhancing ANN capabilities but also provides a novel perspective and methodology for future ANN design.

Spiking Neural Networks (SNNs) and neuromorphic models are more efficient and have more biological realism than the activation functions typically used in deep neural networks, transformer models and generative AI. SNNs have local learning rules, are able to learn on small data sets, and can adapt through neuromodulation. Although research has shown their advantages, there are still few compelling practical applications, especially at the edge where sensors and actuators need to be processed in a timely fashion. One reason for this might be that SNNs are much more challenging to understand, build, and operate due to their intrinsic properties. For instance, the mathematical foundation involves differential equations rather than basic activation functions. To address these challenges, we have developed CARLsim++. It is an integrated toolbox that enables fast and easy creation of neuromorphic applications. It encapsulates the mathematical intrinsics and low-level C++ programming by providing a graphical user interface for users who do not have a background in software engineering but still want to create neuromorphic models. Developers can easily configure inputs and outputs to devices and robots. These can be accurately simulated before deploying on physical devices. CARLsim++ can lead to rapid development of neuromorphic applications for simulation or edge processing.

Beyond providing accurate movements, achieving smooth motion trajectories is a long-standing goal of robotics control theory for arms aiming to replicate natural human movements. Drawing inspiration from biological agents, whose reaching control networks effortlessly give rise to smooth and precise movements, can simplify these control objectives for robot arms. Neuromorphic processors, which mimic the brain's computational principles, are an ideal platform to approximate the accuracy and smoothness of biological controllers while maximizing their energy efficiency and robustness. However, the incompatibility of conventional control methods with neuromorphic hardware limits the computational efficiency and explainability of their existing adaptations. In contrast, the neuronal subnetworks underlying smooth and accurate reaching movements are effective, minimal, and inherently compatible with neuromorphic hardware. In this work, we emulate these networks with a biologically realistic spiking neural network for motor control on neuromorphic hardware. The proposed controller incorporates experimentally-identified short-term synaptic plasticity and specialized neurons that regulate sensory feedback gain to provide smooth and accurate joint control across a wide motion range. Concurrently, it preserves the minimal complexity of its biological counterpart and is directly deployable on Intel's neuromorphic processor. Using the joint controller as a building block and inspired by joint coordination in human arms, we scaled up this approach to control real-world robot arms. The trajectories and smooth, bell-shaped velocity profiles of the resulting motions resembled those of humans, verifying the biological relevance of the controller. Notably, the method achieved state-of-the-art control performance while decreasing the motion jerk by 19% to improve motion smoothness.

New AI'smart' cycling shorts equipped with sensors and wires that emit electrical currents into rider's muscles to improve performance have been unveiled by a UK start-up. While technology had previously been used to enhance other areas of cycling equipment, riding shorts have remained largely unchanged, except for perhaps additional padding and improved materials. But now, a British start-up called Impulse has developed a pair of riding shorts that employ electoral sensors and artificial intelligence to shape and stimulate cyclists' muscles while they are on the move, The Times has reported. The company also plans to utilise the same technology for other activities too, such as for runners and gym-goers. Pictured: A graphic showing how the Impulse smart shorts work, employing sensors, AI and electrical currents that stimulate the rider's muscles to improve cycling performance Devon Lewis (pictured left), a PHD student in neuroscience at the University of Southampton, designed the smart shorts (pictured right) that emit tiny electric currents to improve a rider's performance Devon Lewis, a PHD student in neuroscience at the University of Southampton, designed the shorts that emit tiny electric current into the wearer's hamstring muscles and quads to improve their cycling technique.

Legged locomotion is a challenging task in the field of robotics but a rather simple one in nature. This motivates the use of biological methodologies as solutions to this problem. Central pattern generators are neural networks that are thought to be responsible for locomotion in humans and some animal species. As for robotics, many attempts were made to reproduce such systems and use them for a similar goal. One interesting design model is based on spiking neural networks. This model is the main focus of this work, as its contribution is not limited to engineering but also applicable to neuroscience. This paper introduces a new general framework for building central pattern generators that are task-independent, biologically plausible, and rely on learning methods. The abilities and properties of the presented approach are not only evaluated in simulation but also in a robotic experiment. The results are very promising as the used robot was able to perform stable walking at different speeds and to change speed within the same gait cycle.