De-Sheng Chen,* Tong-Fu Wang,* Jia-Wang Zhu, Bo Zhu, Zeng-Liang Wang, Jian-Gang Cao, Cai-Hong Feng, Jun-Wei Zhao Department of Sports Medicine and Arthroscopy, Tianjin Hospital of Tianjin University, Tianjin, People's Republic of China *These authors contributed equally to this work Correspondence: Jia-Wang Zhu Department of Sports Medicine and Arthroscopy, Tianjin Hospital of Tianjin University, Tianjin, People's Republic of China Email [email protected] Purpose: We aim to present an unsupervised machine learning application in anterior cruciate ligament (ACL) rupture and evaluate whether supervised machine learning-derived radiomics features enable prediction of ACL rupture accurately. Patients and Methods: Sixty-eight patients were reviewed. Their demographic features were recorded, radiomics features were extracted, and the input dataset was defined as a collection of demographic features and radiomics features. The input dataset was automatically classified by the unsupervised machine learning algorithm. Then, we used a supervised machine learning algorithm to construct a radiomics model.