Fundamental breakthroughs across many scientific disciplines are becoming increasingly rare (1). At the same time, challenges related to the reproducibility and scalability of experiments, especially in the natural sciences (2,3), remain significant obstacles. For years, automating scientific experiments has been viewed as the key to solving this problem. However, existing solutions are often rigid and complex, designed to address specific experimental tasks with little adaptability to protocol changes. With advancements in robotics and artificial intelligence, new possibilities are emerging to tackle this challenge in a more flexible and human-centric manner.

Traditional manufacturing faces challenges adapting to dynamic environments and quickly responding to manufacturing changes. The use of multi-agent systems has improved adaptability and coordination but requires further advancements in rapid human instruction comprehension, operational adaptability, and coordination through natural language integration. Large language models like GPT-3.5 and GPT-4 enhance multi-agent manufacturing systems by enabling agents to communicate in natural language and interpret human instructions for decision-making. This research introduces a novel framework where large language models enhance the capabilities of agents in manufacturing, making them more adaptable, and capable of processing context-specific instructions. A case study demonstrates the practical application of this framework, showing how agents can effectively communicate, understand tasks, and execute manufacturing processes, including precise G-code allocation among agents. The findings highlight the importance of continuous large language model integration into multi-agent manufacturing systems and the development of sophisticated agent communication protocols for a more flexible manufacturing system.

Improving the overall equipment effectiveness (OEE) of machines on the shop floor is crucial to ensure the productivity and efficiency of manufacturing systems. To achieve the goal of increased OEE, there is a need to develop flexible runtime control strategies for the system. Decentralized strategies, such as multi-agent systems, have proven effective in improving system flexibility. However, runtime multi-agent control of complex manufacturing systems can be challenging as the agents require extensive communication and computational efforts to coordinate agent activities. One way to improve communication speed and cooperation capabilities between system agents is by providing a common language between these agents to represent knowledge about system behavior. The integration of ontology into multi-agent systems in manufacturing provides agents with the capability to continuously update and refine their knowledge in a global context. This paper contributes to the design of an ontology for multi-agent systems in manufacturing, introducing an extendable knowledge base and a methodology for continuously updating the production data by agents during runtime. To demonstrate the effectiveness of the proposed framework, a case study is conducted in a simulated environment, which shows improvements in OEE during runtime.