Reviews: OnACID: Online Analysis of Calcium Imaging Data in Real Time

This paper proposes an online framework for analyzing calcium imaging data. This framework is built upon the popular and now widely used constrained non-negative matrix factorization (CNMF) method for cell segmentation and calcium time-series analysis (Pnevmatikakis, et al., 2016). While the existing CNMF approach is now being used by many labs across the country, I've talked to many neuroscientists that complain that this method cannot be applied to large datasets and thus its application has been limited. This work extends this method to a real-time decoding setting, making it an extremely useful contribution for the neuroscience community. The paper is well written and the results are compelling.