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[Review] CRISPR-Cas: Adapting to change

Science

The arms race between prokaryotes and their perpetually evolving predators has fueled the evolution of a defense arsenal. The so-called CRISPR-Cas systems--clustered regularly interspaced short palindromic repeats and associated proteins--are adaptive immune defense systems found in bacteria and archaea. The recent exponential growth of research in the CRISPR field has led to the discovery of a diverse range of CRISPR-Cas systems and insight into their defense functions. These systems are divided into two major classes and six types. Each system consists of two components: a locus for memory storage (the CRISPR array) and cas genes that encode the machinery driving immunity.


Scientists store a movie in bacterial DNA

Daily Mail - Science & tech

It is a truly viral movie unlike any seen before - and could change the future of computing. Researchers have revealed the first film stored in bacterial DNA, and say it could herald a revolution in digital storage. The tiny movie, consisting of just five frames, shows a galloping thoroughbred mare named Annie G galloping in 1887, and were taken by the pioneering photographer for his photo series titled Human and Animal Locomotion, one of the first motion pictures ever made. The tiny movie, consisting of just five frames, shows a galloping thoroughbred mare named Annie G galloping in 1887. To the left are the original frames.


Structures of the CRISPR genome integration complex

Science

Bacteria have a highly adaptable DNA-detecting and -editing machine called CRISPR-Cas to ward off virus attack. The Cas1-Cas2 integrase, with the help of an accessory protein called IHF (integration host factor), captures foreign DNA motifs into bacterial CRISPR loci. These motifs then act as sensors of any further invaders. By analyzing the integrase complex structure, Wright et al. show how Cas1-Cas2 recognizes the CRISPR array for site-specific integration (see the Perspective by Globus and Qimron). IHF sharply bends DNA, which allows DNA to access two active sites within the integrase complex to ensure sequence specificity for the integration reaction.


CRISPR-Cas{Phi} from huge phages is a hypercompact genome editor

Science

The CRISPR-Cas system, naturally found in many prokaryotes, is widely used for genome editing. CRISPR arrays in the bacterial genome, derived from the genome of invading viruses, are used to generate a CRISPR RNA that guides the Cas enzyme to destroy repeat viral invaders. Recently, an unexpectedly compact CRISPR-Cas system was identified in huge bacteriophages. Pausch et al . show that even though this system lacks commonly found accessory proteins, it is functional. In addition to a CRISPR array, the only component of the system is an enzyme called CasF, which uses the same active site to process transcripts of the CRISPR arrays into CRISPR RNA and to destroy foreign nucleic acids. This system, which is active in human and plant cells, provides a hypercompact addition to the genome-editing toolbox. Science this issue p. [333][1] CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages. CasΦ uses a single active site for both CRISPR RNA (crRNA) processing and crRNA-guided DNA cutting to target foreign nucleic acids. This hypercompact system is active in vitro and in human and plant cells with expanded target recognition capabilities relative to other CRISPR-Cas proteins. Useful for genome editing and DNA detection but with a molecular weight half that of Cas9 and Cas12a genome-editing enzymes, CasΦ offers advantages for cellular delivery that expand the genome editing toolbox. [1]: /lookup/doi/10.1126/science.abb1400


Novel COVID-19 Test Uses CRISPR Technology

#artificialintelligence

Testing for COVID-19 is a critical component in fighting the pandemic. The key is to have accurate and widespread testing. On April 16, 2020, scientists at the University of California San Francisco and Mammoth Biosciences published in Nature Biotechnology a study that uses CRISPR to detect SARS-CoV-2, the coronavirus that causes the COVID-19 disease, in less than an hour. The CRISPR-based diagnostic test for COVID-19 may offer certain advantages over both serology (blood serum) and real-time reverse transcription–polymerase chain reaction (RT-PCR) tests according to the research team. "Although serology tests are rapid and require minimal equipment, their utility may be limited for diagnosis of acute SARS-CoV-2 infection, because it can take several days to weeks following symptom onset for a patient to mount a detectable antibody response," the researchers wrote in the study.